The Team Europe star was the focus for a number of flashpoints as the atmosphere reached boiling point on Saturday, with the crowd screaming 'f*** you, Rory' at the Masters champion.
The Northern Irishman has been involved in several incidents with the heated New York crowd at Bethpage, with his wife Erica reportedly left in tears at the end of play on Saturday evening.
Rory McIlroy's wife, Erica, was reportedly left in tears following a day of vitriolic abuse from American fans at the Ryder Cup. The pair's relationship was targeted throughout the day.
The Northern Irishman was lining up for a putt during his and Shane Lowry's Saturday afternoon fourballs victory over Justin Thomas and Cameron Young when tensions boiled over.
McIlroy was tormented about Erica, his ex-fiancee tennis star Caroline Wozniacki and other aspects of his personal life by fans as he conquered the Bethpage course.